293 human kidney cell line transformed with ad5 dna  (Microbix Inc)

Journal:

Article Title: Genetic Targeting of an Adenovirus Vector via Replacement of the Fiber Protein with the Phage T4 Fibritin

doi: 10.1128/JVI.75.9.4176-4183.2001

Figure Lengend Snippet: Analysis of Ad5LucFF/6H genome structure. (A) DNA isolated from purified Ad5LucFF/6H virions was subjected to restriction enzyme analysis using a number of restriction endonucleases which cleave either the wild-type fiber or the FF/6H gene sequence. Odd-numbered lanes, control Ad5Luc1 DNA; even-numbered lanes, Ad5LucFF/6H DNA. (B) Diagnostic PCR utilizing a pair of primers flanking the fiber gene in the Ad5 genome was employed to show the absence of the wild-type fiber gene sequence in the Ad5LucFF/6H genome. The primers used in this test were designed to amplify both the wild-type fiber gene (1.8 kb) and the FF/6H gene (1.1 kb). Lane 1, PCR product amplified from wild-type Ad5 DNA; lane 2, PCR product amplified from Ad5LucFF/6H DNA; lane M, 1-kb ladder.

Article Snippet: The 293 human kidney cell line transformed with Ad5 DNA was purchased from Microbix (Toronto, Ontario, Canada).

Techniques: Isolation, Purification, Sequencing, Diagnostic Assay, Amplification